Journal: Frontiers in Oncology

Article Title: A novel prognostic signature based on mitochondrial permeability transition-driven necrosis genes for biochemical recurrence prediction in prostate cancer

doi: 10.3389/fonc.2026.1775602

Figure Lengend Snippet: Enrichment analysis and immune-cell infiltration based on risk groups. (A) GSEA analysis showing top 5 pathways between low- and high-risk groups. (B) Heatmap of differences in pathways between low- and high-risk groups. (C) Stacked bar chart showing infiltration proportions of immune cells using ssGSEA. (D) Correlation analysis of FNDC1, S100A8, and TREM2 with differential immune infiltrating cells. (E) Box plot of differences in 7 types of immune cells within the low- and high-risk groups. (F) Correlation analysis between S100A8 and neutrophils. (G) The differences in the scores of dysfunction, exclusion, MSI, and TIDE between low- and high-risk groups. (H) The differences in the expression of immune checkpoint between low- and high-risk groups. Ns, not significant. * indicate p<0.05, ** indicate p<0.01, *** indicate p<0.001, **** indicate p<0.0001.

Article Snippet: The following were the antibody concentrations for western blotting: rabbit polyclonal anti-FNDC1 antibody (1:1000, catalog no. bs-8460R; Bioss, Beijing, China), rabbit polyclonal anti-TREM2 antibody (1:1000, catalog no. HY- P80920 ; MedChemExpress, Monmouth Junction, NJ, USA), and rabbit polyclonal anti-S100A8 antibody (1:1000, catalog no. AF7929; Beyotime, Shanghai, China), and mouse monoclonal anti-GAPDH (1:2000, 60004-1-Ig, Proteintech, China).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: A novel prognostic signature based on mitochondrial permeability transition-driven necrosis genes for biochemical recurrence prediction in prostate cancer

doi: 10.3389/fonc.2026.1775602

Figure Lengend Snippet: Validation of FNDC1, S100A8, and TREM2 expression. (A) The mRNA expression levels of FNDC1, S100A8, and TREM2 in TCGA-PRAD cohort. (B) The mRNA expression levels of FNDC1, S100A8 and TREM2 were confirmed by q-PCR between paratumor(PT) and prostate cancer tissue. (C, D) The protein expression levels of FNDC1, S100A8 and TREM2 were confirmed by western blot between paratumor(PT) and prostate cancer tissue(T). (E) Immunohistochemistry micrographs revealing FNDC1, S100A8 and TREM2 expression between paratumor(PT) and prostate cancer tissue(T). **P< 0.01; ***P< 0.001.

Article Snippet: The following were the antibody concentrations for western blotting: rabbit polyclonal anti-FNDC1 antibody (1:1000, catalog no. bs-8460R; Bioss, Beijing, China), rabbit polyclonal anti-TREM2 antibody (1:1000, catalog no. HY- P80920 ; MedChemExpress, Monmouth Junction, NJ, USA), and rabbit polyclonal anti-S100A8 antibody (1:1000, catalog no. AF7929; Beyotime, Shanghai, China), and mouse monoclonal anti-GAPDH (1:2000, 60004-1-Ig, Proteintech, China).

Techniques: Biomarker Discovery, Expressing, Western Blot, Immunohistochemistry

Journal: Brain, Behavior, & Immunity - Health

Article Title: Increased circulating TREM2 + microglial extracellular vesicles in aged APP/PS1 Alzheimer's disease rats

doi: 10.1016/j.bbih.2026.101261

Figure Lengend Snippet: TMEM119 + /TREM2 + EVs are increased in 15-month-old APP/PS1 rats. A) Example nanoscale flow cytometry scatter plots of TMEM119, TREM2 and dual-positive EVs. B) TMEM119 + /TREM2 + events/μl labelled in 3-, 9-, and 15-month wildtype and APP/PS1 rat plasma assessed using a 2-way ANOVA (genotype ✕ sex). C) Size distribution of TMEM119 +/ TREM2 + EVs based on standardized reference beads. D) Western blot of TMEM119 and TREM2 from, immunoprecipitated TMEM119 EVs, total rat plasma EVs, and whole cell lysate. E) Representative immunofluorescent 20 ✕ images of TMEM119 and TREM2 in 15-month-old APP/PS1 hippocampus. Scale bar indicates 10 μm ∗ Indicates statistical significance ( p < 0.05) Data represent the group mean ± SEM. n = 10-12 males and females per group. LALS, long angle light scatter; SALS, short angle light scatter.

Article Snippet: Membranes were washed for 3 × 10 min with TBST prior to incubation with anti-mouse (TMEM119, ⍺-tubulin) or anti-rabbit (TREM2) HRP-conjugated secondary antibodies (1:10,000, Jackson Laboratories) for 1 h at room temperature.

Techniques: Flow Cytometry, Clinical Proteomics, Western Blot, Immunoprecipitation

Journal: Brain, Behavior, & Immunity - Health

Article Title: Increased circulating TREM2 + microglial extracellular vesicles in aged APP/PS1 Alzheimer's disease rats

doi: 10.1016/j.bbih.2026.101261

Figure Lengend Snippet: TREM2 mRNA and protein levels differ by age and genotype in the hippocampus. A) Coronal section for the hippocampal region of interest. B) qPCR measurement of TMEM119 and TREM2 in 3-, 9-, and 15-month WT and APP/PS1 hippocampal RNA isolates assessed using 2-way ANOVAs (age ✕ genotype). C-E) Western blot of TMEM119 and TREM2 and corresponding quantification in hippocampal protein isolates from 3-, 9-, and 15-month WT and APP/PS1 rats assessed using t -tests. ∗ Indicates statistical significance ( p < 0.05). Data represent the group mean ± SEM. n = 2-3 males and 2-3 females per group.

Article Snippet: Membranes were washed for 3 × 10 min with TBST prior to incubation with anti-mouse (TMEM119, ⍺-tubulin) or anti-rabbit (TREM2) HRP-conjugated secondary antibodies (1:10,000, Jackson Laboratories) for 1 h at room temperature.

Techniques: Western Blot

Journal: Brain, Behavior, & Immunity - Health

Article Title: Increased circulating TREM2 + microglial extracellular vesicles in aged APP/PS1 Alzheimer's disease rats

doi: 10.1016/j.bbih.2026.101261

Figure Lengend Snippet: Increased TREM2 mRNA in the corpus callosum of 15-month-old rats. A) Coronal section for the corpus callosum region of interest. B) qPCR measurement of TMEM119 and TREM2 in 3-, 9-, and 15-month WT and APP/PS1 corpus callosum RNA isolates assessed using 2-way ANOVAs (age ✕ genotype). C-E) Western blot of TMEM119 and TREM2 and corresponding quantification in corpus callosum protein isolates from 3-, 9-, and 15-month WT and APP/PS1 rats assessed using t -tests. ∗ Indicates statistical significance ( p < 0.05). Data represent the group mean ± SEM. n = 2-3 males and 2-3 females per group.

Article Snippet: Membranes were washed for 3 × 10 min with TBST prior to incubation with anti-mouse (TMEM119, ⍺-tubulin) or anti-rabbit (TREM2) HRP-conjugated secondary antibodies (1:10,000, Jackson Laboratories) for 1 h at room temperature.

Techniques: Western Blot

Journal: Brain, Behavior, & Immunity - Health

Article Title: Increased circulating TREM2 + microglial extracellular vesicles in aged APP/PS1 Alzheimer's disease rats

doi: 10.1016/j.bbih.2026.101261

Figure Lengend Snippet: Increased histological TREM2 expression in aged APP/PS1 rats. A) Quantification of TREM2 cell density in the hippocampus of 3-, 9-, and 15-month WT and APP/PS1 rats assessed using t -tests. B) Representative 20 ✕ images of TREM2 + cells in the hippocampus of 15-month WT and APP/PS1 rats. C) Quantification of TREM2 cell density in the corpus callosum of 3-, 9-, and 15-month WT and APP/PS1 rats assessed using t -tests. D) Representative 20 ✕ images of TREM2 + cells in the corpus callosum of 15-month WT and APP/PS1 rats. Scale bar indicates 25 μm ∗ Indicates statistical significance ( p < 0.05). Data represent the group mean ± SEM. n = 4 males and 4 females per group.

Article Snippet: Membranes were washed for 3 × 10 min with TBST prior to incubation with anti-mouse (TMEM119, ⍺-tubulin) or anti-rabbit (TREM2) HRP-conjugated secondary antibodies (1:10,000, Jackson Laboratories) for 1 h at room temperature.

Techniques: Expressing

Journal: Brain, Behavior, & Immunity - Health

Article Title: Increased circulating TREM2 + microglial extracellular vesicles in aged APP/PS1 Alzheimer's disease rats

doi: 10.1016/j.bbih.2026.101261

Figure Lengend Snippet: Impaired spatial memory associates with increased TMEM119 + /TREM2 + EVs in 15-month-old rats. A) Simple linear regression between TMEM119 + /TREM2 + events/μl and total RAWM errors in 3-, 9-, and 15-month WT and APP/PS1 rats. n = 10-12 males and females per group.

Article Snippet: Membranes were washed for 3 × 10 min with TBST prior to incubation with anti-mouse (TMEM119, ⍺-tubulin) or anti-rabbit (TREM2) HRP-conjugated secondary antibodies (1:10,000, Jackson Laboratories) for 1 h at room temperature.

Techniques: